A Serosurvey of Japanese Encephalitis Virus in Monkeys and Humans Living in Proximity in Thailand (2023)

Medecine, Nanthanida Mongkol, Scopus

Title           : A Serosurvey of Japanese Encephalitis Virus in Monkeys and Humans Living in Proximity in Thailand

Researcher       :  Divya Lakhotia, Yin May Tun, Nanthanida Mongkol, Oranit Likhit, Sarocha Suthisawat, Suthee Mangmee, Daraka Tongthainan, Wirasak Fungfuang, Phitsanu Tulayakul and Kobporn Boonnak

 Abstract            :  Japanese encephalitis virus (JEV) is a member of the Flaviviridae family and one of Asia’s most common causes of encephalitis. JEV is a zoonotic virus that is transmitted to humans through the bite of infected mosquitoes of the Culex species. While humans are dead-end hosts for the virus, domestic animals such as pigs and birds are amplification hosts. Although JEV naturally infected monkeys have been reported in Asia, the role of non-human primates (NHPs) in the JEV transmission cycle has not been intensively investigated. In this study, we demonstrated neutralizing antibodies against JEV in NHPs (Macaca fascicularis) and humans living in proximity in two provinces located in western and eastern Thailand by using Plaque Reduction Neutralization Test (PRNT). We found a 14.7% and 5.6% seropositive rate in monkeys and 43.7% and 45.2% seropositive rate in humans living in west and east Thailand, respectively. This study observed a higher seropositivity rate in the older age group in humans. The presence of JEV neutralizing antibodies in NHPs that live in proximity to humans shows the occurrence of natural JEV infection, suggesting the endemic transmission of this virus in NHPs. According to the One Health concept, regular serological studies should be conducted especially at the animal–human interface.

Keywords:  Japanese encephalitis virus; seroprevalence; Plaque Reduction Neutralization Test; Flavivirus

Link to article :  Viruses 2023, 15(5), 1125. https://doi.org/10.3390/v15051125

Journal : Viruses / in Scopus

Citation    : Lakhotia, D., Tun, Y. M., Mongkol, N., Likhit, O., Suthisawat, S., Mangmee, S., Tongthainan, D., Fungfuang, W., Tulayakul, P., & Boonnak, K. (2023). A Serosurvey of Japanese Encephalitis Virus in Monkeys and Humans Living in Proximity in Thailand. Viruses, 15(5), 1125. https://doi.org/10.3390/v15051125

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A Shear-Thinning, Self-Healing, Dual-Cross Linked Hydrogel Based on Gelatin/Vanillin/Fe3+/AGP-AgNPs: Synthesis, Antibacterial, and Wound-Healing Assessment (2023)

Medecine, Sarawut Lapmanee, Scopus

Title           :  A Shear-Thinning, Self-Healing, Dual-Cross Linked Hydrogel Based on Gelatin/Vanillin/Fe3+/AGP-AgNPs: Synthesis, Antibacterial, and Wound-Healing Assessment

Researcher       : Chanon Talodthaisong,  Rina Patramanon,  Saengrawee Thammawithan, Sarawut Lapmanee, Lamai Maikaeo,  Phitchan Sricharoen,  Mattaka Khongkow,  Katawut Namdee,  Angkana Jantimaporn,  Navaphun Kayunkid,  James A. Hutchison,  Sirinan Kulchat

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article : Macromolecular Bioscience, 2023, 23(12), 2300250.  https://doi.org/10.1002/mabi.202300250

Citation  :  Talodthaisong, C., Patramanon, R., Thammawithan, S., Lapmanee, S., Maikaeo, L., Sricharoen, P., Khongkow, M., Namdee, K.,    Jantimaporn, A., Kayunkid, N., Hutchison, J. A., & Kulchat, S. (2023). A Shear-Thinning, Self-Healing, Dual-Cross Linked Hydrogel Based on Gelatin/Vanillin/Fe3+/AGP-AgNPs: Synthesis, Antibacterial, and Wound-Healing Assessment. Macromolecular Bioscience, 23(12), 2300250.  https://doi.org/10.1002/mabi.202300250

Journal : Macromolecular Bioscience / in Scopus

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Atomoxetine and escitalopram migrate the derangement of the temporomandibular joint morphologic and histologic changes in rats exposed to stress-induced depression (2023)

Medecine, Sakkarin Bhubhanil, Sarawut Lapmanee, Scopus

Title           :  Atomoxetine and escitalopram migrate the derangement of the temporomandibular joint morphologic and histologic changes in rats exposed to stress-induced depression

Researcher       :  Thanatta Songphaeng, Sarawut Lapmanee, Sakkarin Bhubhanil, Kamonchanok Momdee, Catleya Rojviriya, Kemporn Kitsahawong, Pattama Chailertvanitkul, Jariya U. Welbat, Supawich Morkmued

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article :  Journal of Oral Science, 2023, 65(4), pp. 219–225. https://doi.org/10.2334/josnusd.23-0077

Citation  :   Songphaeng, T., Lapmanee, S., Bhubhanil, S., Momdee,  K., Rojviriya, C., Kitsahawong, K., Chailertvanitkul, P., Welbat, J. U., & Morkmued, S. (2023). Atomoxetine and escitalopram migrate the derangement of the temporomandibular joint morphologic and histologic changes in rats exposed to stress-induced depression. Journal of Oral Science, 65(4), 219–225. https://doi.org/10.2334/josnusd.23-0077

Journal : Journal of Oral Science/ in Scopus

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Clinical and molecular characteristics of FMR1 microdeletion in patient with fragile X syndrome and review of the literature (2024)

Medecine, Pornprot Limprasert, Scopus

Title           : Clinical and molecular characteristics of FMR1 microdeletion in patient with fragile X syndrome and review of the literature

Researcher       :  Areerat Hnoonual, Oradawan Plong-On, Juthamas Worachotekamjorn, Chariyawan Charalsawadi, Pornprot Limprasert

 Abstract            :  Background: Fragile X syndrome (FXS) is mainly caused by FMR1 CGG repeat expansions. Other types of mutations, particularly deletions, are also responsible for FXS phenotypes, however these mutations are often missed by routine clinical testing.

Materials and methods: Molecular diagnosis in cases of suspected FXS was a combination of PCR and Southern blot. Measurement of the FMRP protein level was useful for detecting potentially deleterious impact.

Results: PCR analysis and Southern blot revealed a case with premutation and suspected deletion alleles. Sanger sequencing showed that the deletion involved 313 bp upstream of repeats and some parts of CGG repeat tract, leaving transcription start site. FMRP was detected in 5.5 % of blood lymphocytes.

Conclusion: According to our review of case reports, most patients carrying microdeletion and full mutation had typical features of FXS. To our knowledge, our case is the first to describe mosaicism of a premutation and microdeletion in the FMR1 gene. The patient was probably protected from the effects of the deletion by mosaicism with premutation allele, leading to milder phenotype. It is thus important to consider appropriate techniques for detecting FMR1 variants other than repeat expansions which cannot be detected by routine FXS diagnosis.

Keywords: Deletion; FMR1; Fragile X syndrome; Mosaicism; Premutation.

Link to article : Clinica Chimica Acta, 2024, 553, 117728. https://doi.org/10.1016/j.cca.2023.117728

Journal : Clinica Chimica Acta / in Scopus

Citation    : Hnoonual, A., Plong-On, O., Worachotekamjorn, J., Charalsawadi, C., Limprasert, P. (2024). Clinical and molecular characteristics of FMR1 microdeletion in patient with fragile X syndrome and review of the literature. Clinica Chimica Acta, 553, 117728. https://doi.org/10.1016/j.cca.2023.117728

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Construction of the Short-Form Thai-Home Fall Hazard Assessment Tool (Thai-HFHAT-SF) and Testing Its Validity and Reliability in the Elderly (2022)

Medecine, Sarawut Lapmanee, Scopus

Title           :  Construction of the Short-Form Thai-Home Fall Hazard Assessment Tool (Thai-HFHAT-SF) and Testing Its Validity and Reliability in the Elderly

Researcher       :  Charupa Lektip, Sarawut Lapmanee, Rewwadee Petsirasan, Kanda Chaipinyo, Saifon Lektip, and Jiraphat Nawarat.

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article : International Journal of Environmental Research and Public Health, 2022,19(9), 5187. https://doi.org/10.3390/ijerph19095187

Citation  :  Lektip, C., Lapmanee, S., Petsirasan, R., Chaipinyo, K., Lektip, S., & Nawarat, J. (2022). Construction of the Short-Form Thai-Home Fall Hazard Assessment Tool (Thai-HFHAT-SF) and Testing Its Validity and Reliability in the Elderly. International Journal of Environmental Research and Public Health, 19(9), 5187. https://doi.org/10.3390/ijerph19095187

Journal : International Journal of Environmental Research and Public Health / in Scopus

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Effects of scapulothoracic exercises on chest mobility, respiratory muscle strength, and pulmonary function in male COPD patients with forward shoulder posture: a randomized control trial (2022)

Medecine, Sarawut Lapmanee, Scopus

Title           :  Effects of scapulothoracic exercises on chest mobility, respiratory muscle strength, and pulmonary function in male COPD patients with forward shoulder posture: a randomized control trial

Researcher       : Kanogwun Thongchote, Usa Chinwaro, Sarawut Lapmanee

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article : F1000Research 2022, 11, 1284 https://doi.org/10.12688/f1000research.126832.1

Citation  :  Thongchote, K., Chinwaro, U., & Lapmanee, S. (2022). Effects of scapulothoracic exercises on chest mobility, respiratory muscle strength, and pulmonary function in male COPD patients with forward shoulder posture: a randomized control trial. F1000Research , 11, 1284. https://doi.org/10.12688/f1000research.126832.1

Journal :  F1000Research / in Scopus

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Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels (2021)

Medecine, Sakkarin Bhubhanil, Sarawut Lapmanee, Scopus

Title           :  Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

Researcher       : Sakkarin Bhubhanil, Chanon Talodthaisong, Mattaka Khongkow, Katawut Namdee, Prapimpun Wongchitrat, Werayut Yingmema, James A. Hutchison, Sarawut Lapmanee & Sirinan Kulchat

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article : Scientific Reports, 2021, 11, 21836. https://doi.org/10.1038/s41598-021-01262-x

Citation  : Bhubhanil, S., Talodthaisong, C., Khongkow, M.,Namdee,  K., Wongchitrat, P.,Yingmema, W.,  Hutchison, J. A., Lapmanee S., & Kulchat, S. (2021). Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels. Scientific Reports, 11, 21836  https://doi.org/10.1038/s41598-021-01262-x

Journal : Scientific Reports / in Scopus

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Factors Affecting Research Conduct and Publication Among Thai Medical Students in University-Affiliated Medical Schools (2024)

Medecine, Sarawut Lapmanee, Scopus

Title           :  Factors Affecting Research Conduct and Publication Among Thai Medical Students in University-Affiliated Medical Schools

Researcher       :  Thana Khawcharoenporn, Sumalee Kondo, Naesinee Chaiear, Krishna Suvarnabhumi & Sarawut Lapmanee

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article :  Journal of Academic Ethics DOI: https://doi.org/10.1007/s10805-023-09500-9

Citation  :  Khawcharoenporn, T., Kondo, S., Chaiear, N., Suvarnabhumi, K., & Lapmanee, S. (2024). Factors Affecting Research Conduct and Publication Among Thai Medical Students in University-Affiliated Medical Schools. Journal of Academic Ethics, (2024). https://doi.org/10.1007/s10805-023-09500-9

Journal : Journal of Academic Ethics  / in Scopus

ฐานข้อมูลงานวิจัย มหาวิทยาลัยสยาม  :  https://e-research.siam.edu/kb/factors-affecting-research-conduct/

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Genetic characterization of carbapenem-resistant enterobacteriaceae and the spread of carbapenem-resistant klebsiella pneumonia ST340 at a university hospital in Thailand (2015)

Medecine, Scopus, Thidarat Netikul

Title           : Genetic characterization of carbapenem-resistant enterobacteriaceae and the spread of carbapenem-resistant klebsiella pneumonia ST340 at a university hospital in Thailand

Researcher       : Netikul, T.,[mfn]1[/mfn] Kiratisin, P.

Department     : 1Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : med@siam.edu

Abstract            : Carbapenem-resistant Enterobacteriaceae (CRE) has increasingly spread worldwide in the past decade. The prevalence and characteristics of CRE in Thailand are unknown. In this study, we conducted a 2-year surveillance of CRE among 12,741 clinical isolates of Enterobacteriaceae at the largest university hospital in Thailand with molecular characterization of beta-lactamase (bla) genes, including carbapenemase genes. The CRE prevalence was 1.4%. blaKPC-13 and blaIMP-14a were the only carbapenemase genes detected among these CRE isolates. blaKPC-13 gene was found in a single isolate of Escherichia coli, Enterobacter cloacae and Citrobacter freundii, and blaIMP-14a was found in four isolates of Klebsiella pneumoniae. Carbapenem-resistant K. pneumoniae (CRKP) isolates were resistant to multiple carbapenems at a higher ratio than other CRE species, and thus were further characterized for resistance phenotypes, bla genotypes and molecular epidemiology. Most CRKP isolates harboured multiple bla genes, especially those related to extended-spectrum beta-lactamases. Seven CRKP isolates were resistant to all tested carbapenems, and showed decreased ompK35 and/or ompK36 porin gene expression. Molecular typing of CRKP based on pulsed-field gel electrophoresis (PFGE) demonstrated several unrelated clones. Multilocus sequence typing (MLST) was partially concordant with PFGE results and revealed that ST340, a member of drug-resistant K. pneumoniae clonal complex 258, was the most predominant clone, followed by ST48, ST11 and ST273. The novel ST1645 was identified from this study. ST340 has neither been shown to be predominated among CRKP from other studies, nor been reported in Thailand. Therefore, it emphases a critical concern to monitor and control the spread of CRKP.

Link to Academic article:  https://doi.org/10.1371/journal.pone.0139116

Journal : PLoS ONE, 2015, 10(9), e0139116

Bibliography     : Netikul, T., & Kiratisin, P. (2015). Genetic characterization of carbapenem-resistant enterobacteriaceae and the spread of carbapenem-resistant klebsiella pneumonia ST340 at a university hospital in Thailand. PLoS ONE, 2015, 10(9), e0139116. https://doi.org/10.1371/journal.pone.0139116

 

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Home hazard modification programs for reducing falls in older adults: a systematic review and meta-analysis (2023)

Medecine, Sarawut Lapmanee, Scopus

Title           :  Home hazard modification programs for reducing falls in older adults: a systematic review and meta-analysis

Researcher       :  Charupa Lektip, Sirawee Chaovalit, Apichai Wattanapisit, Sarawut Lapmanee, Jiraphat Nawarat, Weeranan Yaemrattanakul

Department     : Faculty of Medicine, Siam University, Bangkok, Thailand

E-mail                : sarawut.lap@siam.ed

Link to article :  PeerJ, 11, e15699. https://doi.org/10.7717/peerj.15699

Citation  :  Lektip, C., Chaovalit, S., Wattanapisit, A., Lapmanee, S., Nawarat. J., & Yaemrattanakul, W. (2023). Home hazard modification programs for reducing falls in older adults: a systematic review and meta-analysis. PeerJ, 11, e15699. https://doi.org/10.7717/peerj.15699

Journal :   PeerJ / in Scopus

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