Title           :  Bioactive compound and chemical characterization of lactic acid bacteria from fermented food as bio-preservative agents to control food-borne pathogens | Compuestos bioactivos y caracterización química de bacterias lácticas de alimentos fermentados como agentes bioconservantes para el control de patógenos alimentarios

Researcher       : Techaoei, S., Jarmkom, K., Dumrongphuttidecha, T., Khobjai, W.

Department     :  Faculty of Medicine, Siam University, Bangkok, Thailand

Abstract            :  Context: Lactic acid bacteria (LAB), which are found in many fermented foods, are known to produce antimicrobial compounds that play a vital role in food bio-preservation.

Aims: To screen, identify, characterize, and determine the secondary metabolites of LAB isolated from Thai fermented foods that are beneficial against Escherichia coli and Staphylococcus aureus.

Methods: The antimicrobial activity of cell free supernatant (CFS) was evaluated by agar well diffusion assay, and determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC). Bacterial strains were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and chemical compound was conducted by gas chromatography and mass spectrometry (GC-MS). Additionally, microbial dynamic and bile salt tolerance were assessed.

Results: Seven of the 90 lactic acid isolates from Thai fermented foods, showed antibacterial activity against E. coli and S. aureus in the 12.00–16.00 mm inhibitory zone. It was identified that the bacteria were Lactobacillus pentosus, Lactobacillus farciminis, Lactobacillus brevis, and Lactobacillus plantarum. The best antibacterial activity was represented by LBST1861 strain, which also provided bile salt resistance at 0.3% for 24 hours and had MIC and MBC values of 12.5 mg/mL and 50.0 mg/mL against S. aureus and E. coli, respectively. Furthermore, the GC-MS discovered a total of 16 chemical compounds that may be used to limit microbial growth and has a potential to be employed as a bio-preservative.

Conclusions: The most potent strain of LBST1861 strain against S. aureus and E. coli as L. plantarum, isolated from fermented foods in Thailand, generated significant bioactive chemicals that can be applied to promote food products.

Keywords: antimicrobial activity; bioactive compounds; fermented foods; lactic acid bacteria; probiotics.

Link to article: Journal of Pharmacy and Pharmacognosy Research, 2023, 11(6), pp. 1044–1055. https://doi.org/10.56499/jppres23.1713_11.6.1044

Journal : Journal of Pharmacy and Pharmacognosy Research / in Scopus

Citation : Techaoei, S., Jarmkom, K., Dumrongphuttidecha, T., & Khobjai, W. (2023). Bioactive compound and chemical characterization of lactic acid bacteria from fermented food as bio-preservative agents to control food-borne pathogens | Compuestos bioactivos y caracterización química de bacterias lácticas de alimentos fermentados como agentes bioconservantes para el control de patógenos alimentarios. Journal of Pharmacy and Pharmacognosy Research, 11(6), 1044–1055. https://doi.org/10.56499/jppres23.1713_11.6.1044

Title           : Bioactivities of karanda (Carissa carandas Linn.) fruit extracts for novel cosmeceutical applications

Researcher       :  Monsicha Khuanekkaphan¹, Warachate Khobjai², Chanai Noysang³, Nakuntwalai Wisidsri¹, Suradwadee Thungmungmee³

Department     : ¹ Department of Health and Aesthetics, Thai Traditional Medicine College, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand
² Department of Clinical Chemistry, Faculty of Medical Technology, Nation University, Lampang, Thailand
³ Department of Innovation of Health Products, Thai Traditional Medicine College, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand

Abstract            :  The aim of this research was to determine the total phenolic content (TPC), antioxidation, antiaging, and antibacterial activities of Carissa carandas Linn., and aims at the novel plant sources which is utilized for their cosmeceutical applications. The two conditions (fresh and dried) and three stages (unripe, ripe, and fully ripe) of C. carandas were extracted by ethanolic maceration. Folin–Ciocalteu assay was used for determining the TPC. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used for estimating antioxidant activity. The inhibitory tyrosinase activities were measured using the modified dopachrome assay. Antiaging was evaluated by inhibition of collagenase and elastase, and antibacterial activities. The result of six extracts from C. carandas showed that the highest phenolic content and elastase inhibition of the fresh fruit in fully ripe stage were 100.31 ± 2.64 mg GAE/g extract and 14.11% ± 0.95%, respectively. The fresh fruit in the unripe stage showed that the strongest percentage of DPPH IC₅₀ and collagenase inhibitory activity were 29.11 ± 0.23 μg/mL and 85.94% ± 2.21%, respectively. The ethanolic extract of unripe dried fruit exhibited the highest antioxidant activity in the of ABTS assay, with an IC₅₀ of 0.17 ± 0.01 μg/mL. The MBC displayed the dried fruit ripe stage anti Cutibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus strains were 25.0, 25.0, and 16.25 mg/mL, respectively. The fresh fruit in the ripe stage showed that the strongest inhibition tyrosinase was 93.88% ± 5.64%. The conclusion of this research indicates that the fresh fruit of C. carandas fruit extracts has high potential as a novel cosmeceuticals’ applications to antiaging and skin whitening. The dried fruit in ripe stage extract has the most effective ingredient for antiacne products.

Keywords: Antiaging, antibacterial, Carissa carandas, cosmeceuticals, karanda

Link to Academic article: DOI: 10.4103/japtr.JAPTR_254_20

Journal : Journal of Advanced Pharmaceutical Technology and Research, 2021, 12(2)

Bibliography     :  Khuanekkaphan, M., Khobjai, W., Noysang, C., Wisidsri, N., & Thungmungmee, S. (2021). Bioactivities of karanda (Carissa carandas Linn.) fruit extracts for novel cosmeceutical applications. J Adv Pharm Technol Res, 12(2) 162-168. Retrieved from https://www.japtr.org/text.asp?2021/12/2/162/314675

Title           : Improvement of organic red pigment prodcution by Monascus Purpureus TISTR3651 using pathumthani-1 rice-based medium in submerged and solid-state fermentation

Researcher       : Techaoei, S., Jarmkom, K., Dumrongphuttidacha, T., Khobjai, W.

Department     :

Abstract            :  Objective: This research is to study the production of natural red pigment by Monascus purpureus TISTR3615 in the submerged and solid-state
fermentation system using Pathumthani-1 rice as a carbon source.
Methods: The antioxidant activity of the red pigment was evaluated in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS radical scavenging assay,
and ferric-reducing antioxidant power (FRAP) assay, including total phenolic compound.
Results and Discussion: The maximum of red pigment production was 0.55±0.02/ml (OD 680 nm) after incubation at 30°C for 24 days. The
antioxidant activity based on inhibition DPPH (%), ABTS radical scavenging activity (%), and FRAP activity (mM Fe2+/g) was 97.80±1.51,
68.64±0.46, and 0.32±0.021, respectively. The total phenolic content was 164.78±2.82 μg GAE/mg.
Conclusion: It was estimated that Monascus pigments, leading to nutraceutical and pharmaceutical applications, cosmetic industry, and food
industry.

Keywords: Natural pigment, Monascus purpureus, Submerged fermentation, Solid-state fermentation, Agricultural product

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0103

Journal : International Journal of Applied Pharmaceutics, 2021, 13(Special issue 1)

Bibliography     :  Techaoei, S., Jarmkom, K., Dumrongphuttidacha, T., & Khobjai, W. (2021). Improvement of organic red pigment prodcution by Monascus Purpureus TISTR3651 using pathumthani-1 rice-based medium in submerged and solid-state fermentation. International Journal of Applied Pharmaceutics, 13(Special issue 1), 47–50. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0103

Title           : In vitro antiplatelet and anticoagulant activity of indigenous vegetables from Southern Thailand

Researcher       : Sukati, S., Khobjai, W.

Department     :

Abstract            :  Objective: Epidemiological studies have indicated that diets rich in fruits and vegetables help reduce the risk of cardiovascular diseases (CVDs). However, data about the antithrombotic activity of local vegetables is rare. The objective of this study was to evaluate antiplatelet and anticoagulant activity in indigenous vegetables with high phenolic compounds collected from Southern Thailand.
Methods: Five selected indigenous vegetables were crudely extracted by distilled water and 80% methanol. The extracts were screened for in vitro antiplatelet and anticoagulant activity at a concentration of 10 μg/μl. The antiplatelet activity was measured by inhibition of platelet adhesion to collagen and thrombin-induced platelet aggregation, while the anticoagulant activity was assessed by the prothrombin time (PT) and activated partial thromboplastin time (APTT) tests.
Results: Among the selected vegetables, the extracts of mon-pu (Glochidion perakense Hook.f.) and young cashew leaves (Anacardium occidentale L.) showed high antithrombotic properties. The highest antithrombotic activity was observed in the methanolic extract of mon-pu, which showed
92.79±0.78% of platelet adhesion inhibition, 102.9±1.53% of platelet aggregation inhibition, and a prolonged APTT assay (48.92±0.94 s). The prolonged APTT but normal PT results suggested that the extract could affect factors VIII, IX, XI, and XII of the intrinsic coagulation pathway.
Conclusion: Our findings demonstrated antiplatelet and anticoagulation properties of indigenous vegetables from Southern Thailand. The multipotential effects of mon-pu extracts on antithrombosis evidently suggest that mon-pu can be considered as an excellent nutraceutical option in the prevention of thrombosis-related CVDs caused by different mechanisms.

Keywords: Indigenous vegetables, Antiplatelet activity, Anticoagulant activity, Southern Thailand

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0100

Journal : International Journal of Applied Pharmaceutics, 2021, 13(Special issue 1)

Bibliography     : Sukati, S., & Khobjai, W. (2021). In vitro antiplatelet and anticoagulant activity of indigenous vegetables from Southern Thailand. International Journal of Applied Pharmaceutics, 13(Special Issue 1), 38–42. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0100

Title           : Phytonutrient screening by gas chromatography–mass spectrometry and anti-aging properties of glycosmis pentaphylla (Retz.) dc. (som-chuen) extracts

Researcher       : Dumrongphuttidecha, T., Khobjai, W., Techaeoi, S., Jarmkom, K., Thungmungmee, S.

Department     :

Abstract            :  Objective: The aims of this study were to screen the phytonutrient constituents and investigate their anti-aging property of leaves and bark of
Glycosmis pentaphylla (Retz.) DC. (GP).
Methods: GP is the medicinal plant which used as traditional medicine. Glycosmis pentaphylla leaves (GPL) and Glycosmis pentaphylla bark (GPB) of
GP were extracted with ethanol. The gas chromatography–mass spectrometry was used for phytonutrients analysis. The anti-aging properties were
performed using ABTS assay, deoxyribose degradation assay, bovine serum albumin (BSA)-fructose model, and matrix metalloproteinase 1 (MMP-1)
inhibitory activity.
Results: The important phytonutrients of GPL were α-tocopherol, linolenic acid, squalene, stigmasterol, and β-amyrin and for GPB were α-tocopherol,
phytol, campesterol, stigmasterol, dictamine, and γ-sitosterol. The percentage inhibition of GPL and GPB extracts at various concentrations was
between 16.57–76.05 and 20.66-78.81 by ABTS assay, 68.24–90.06 and 73.83–96.64% by deoxyribose degradation assay, 4.24–99.98 and 54.81–
99.94 by BSA-fructose model, and 6.31–81.55 and 1.06–74.45 by MMP-1 inhibitory activity, respectively.
Conclusion: Leaves and bark extracts of GP (Retz.) DC. are good sources of important phytonutrients and have the potential in anti-aging property.

Keywords: Glycosmis pentaphylla (Retz) DC, Anti-aging, Phytonutrients, Anti-Glycation, Matrix Mettalloproteinase-1

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0105

Journal : International Journal of Applied Pharmaceuticsthis, 2021, 13(Special issue 1)

Bibliography     :  Dumrongphuttidecha, T., Khobjai, W., Techaeoi, S., Jarmkom, K., & Thungmungmee, S. (2021). Phytonutrient screening by gas chromatography–mass spectrometry and anti-aging properties of glycosmis pentaphylla (Retz.) dc. (som-chuen) extracts. International Journal of Applied Pharmaceuticsthis, 13(Special Issue 1), 55–58. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0105