Bioactivities of karanda (Carissa carandas Linn.) fruit extracts for novel cosmeceutical applications (2021)

Title           : Bioactivities of karanda (Carissa carandas Linn.) fruit extracts for novel cosmeceutical applications

Researcher       :  Monsicha Khuanekkaphan¹, Warachate Khobjai², Chanai Noysang³, Nakuntwalai Wisidsri¹, Suradwadee Thungmungmee³

Department     : ¹ Department of Health and Aesthetics, Thai Traditional Medicine College, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand
² Department of Clinical Chemistry, Faculty of Medical Technology, Nation University, Lampang, Thailand
³ Department of Innovation of Health Products, Thai Traditional Medicine College, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand

Abstract            :  The aim of this research was to determine the total phenolic content (TPC), antioxidation, antiaging, and antibacterial activities of Carissa carandas Linn., and aims at the novel plant sources which is utilized for their cosmeceutical applications. The two conditions (fresh and dried) and three stages (unripe, ripe, and fully ripe) of C. carandas were extracted by ethanolic maceration. Folin–Ciocalteu assay was used for determining the TPC. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays were used for estimating antioxidant activity. The inhibitory tyrosinase activities were measured using the modified dopachrome assay. Antiaging was evaluated by inhibition of collagenase and elastase, and antibacterial activities. The result of six extracts from C. carandas showed that the highest phenolic content and elastase inhibition of the fresh fruit in fully ripe stage were 100.31 ± 2.64 mg GAE/g extract and 14.11% ± 0.95%, respectively. The fresh fruit in the unripe stage showed that the strongest percentage of DPPH IC₅₀ and collagenase inhibitory activity were 29.11 ± 0.23 μg/mL and 85.94% ± 2.21%, respectively. The ethanolic extract of unripe dried fruit exhibited the highest antioxidant activity in the of ABTS assay, with an IC₅₀ of 0.17 ± 0.01 μg/mL. The MBC displayed the dried fruit ripe stage anti Cutibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus strains were 25.0, 25.0, and 16.25 mg/mL, respectively. The fresh fruit in the ripe stage showed that the strongest inhibition tyrosinase was 93.88% ± 5.64%. The conclusion of this research indicates that the fresh fruit of C. carandas fruit extracts has high potential as a novel cosmeceuticals’ applications to antiaging and skin whitening. The dried fruit in ripe stage extract has the most effective ingredient for antiacne products.

Keywords: Antiaging, antibacterial, Carissa carandas, cosmeceuticals, karanda

Link to Academic article: DOI: 10.4103/japtr.JAPTR_254_20

Journal : Journal of Advanced Pharmaceutical Technology and Research, 2021, 12(2)

Bibliography     :  Khuanekkaphan, M., Khobjai, W., Noysang, C., Wisidsri, N., & Thungmungmee, S. (2021). Bioactivities of karanda (Carissa carandas Linn.) fruit extracts for novel cosmeceutical applications. J Adv Pharm Technol Res, 12(2) 162-168. Retrieved from https://www.japtr.org/text.asp?2021/12/2/162/314675