Aldosterone nongenomically induces angiotensin II receptor dimerization in rat kidney: Role of mineralocorticoid receptor and NADPH oxidase (2019)
Title : Aldosterone nongenomically induces angiotensin II receptor dimerization in rat kidney: Role of mineralocorticoid receptor and NADPH oxidase
Researcher : Sinphitukkul, K., Manotham, K., Eiam-Ong, S., Eiam-Ong, S.
Abstract :
Introduction: Previous in vitro studies demonstrated that aldosterone nongenomically induces transglutaminase (TG) and reactive oxygen species (ROS), which enhanced angiotensin II receptor (ATR) dimerization. There are no in vivo data in the kidney.
Material and methods: Male Wistar rats were intraperitoneally injected with normal saline solution, or aldosterone (Aldo: 150 μg/kg BW); or received pretreatment with eplerenone (mineralocorticoid receptor (MR) blocker, Ep. + Aldo), or with apocynin (nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, Apo. + Aldo) 30 min before aldosterone. Thirty minutes after aldosterone injection, protein abundances of dimeric and monomeric forms of AT1R and AT2R, and protein abundances and localizations of TG2 and p47phox, a cytosolic subunit of NADPH oxidase, were determined by Western blot analysis and immunohistochemistry, respectively.
Results: Protein abundances of dimeric forms of AT1R and AT2R were enhanced by 170% and 70%, respectively. Apocynin could block dimeric forms of both receptors while eplerenone inhibited only AT2R. Monomeric protein levels of both receptors were maintained. Aldosterone significantly enhanced TG2 and p47phox protein abundances, which were blunted by eplerenone or apocynin. Aldosterone stimulated p47phox protein expression in both the cortex and the medulla while TG2 was induced mostly in the medulla. Eplerenone or apocynin normalized the immunoreactivity of both TG2 and p47phox.
Conclusions: This is the first in vivo study demonstrating that aldosterone nongenomically increases renal TG2 and p47phox protein expression and then activates AT1R and AT2R dimerizations. Aldosterone-stimulated AT1R and AT2R dimerizations are mediated through activation of NADPH oxidase. Aldosterone-induced AT1R dimer formation is an MR-independent pathway, whereas the formation of AT2R dimer is modulated in an MR-dependent manner.
Keywords: NADPH oxidase; aldosterone; angiotensin II receptor dimerization; mineralocorticoid receptor; nongenomic action; rat kidney.
Link to Academic article: DOI: 10.5114/aoms.2019.87135
Journal : ,
Bibliography : Sinphitukkul, K., Manotham, K., Eiam-Ong, S., & Eiam-Ong, S. (2018). Aldosterone nongenomically induces angiotensin II receptor dimerization in rat kidney: role of mineralocorticoid receptor and NADPH oxidase.
Association of HLA genotypes with Beta-lactam antibiotic hypersensitivity in children (2019)
Title : Association of HLA genotypes with Beta-lactam antibiotic hypersensitivity in children
Researcher : Clin.Prof.Suwat Benjaponpitak
Department : Faculty of Medicine, Siam University, Bangkok, Thailand
E-mail : med@siam.edu
Abstract : Background: Beta-lactam (BL) antibiotics hypersensitivity is common in children. Clinical manifestation of BL hypersensitivity varies from mild to severe cutaneous adverse drug reactions (SCARs).
Objective: To determine the association of HLA genotype and BL hypersensitivity and the prevalence of true drug allergy in patients with history of BL hypersensitivity.
Methods: A case-control study was performed in 117 children with aged 1-18 years. Children with history of non-SCARs BL hypersensitivity were evaluated for true drug hypersensitivity including skin test and drug provocation test. Tolerant control patients were children who could tolerate BL for at least 7 days without hypersensitivity reaction. HLA genotype (HLA-A, HLA-B, HLA-C and HLA-DRB1) were performed in 24 cases and 93 tolerant controls using PCR-SSO (polymerase chain reaction – sequence specific oligonucleotide probes).
Results: There were association of HLA-C*04:06 (OR = 13.14, 95%CI: 1.3-137.71; p = 0.027), and HLA-C*08:01 (OR = 4.83, 95%CI: 1.93-16.70; p = 0.016) with BL hypersensitivity. HLA-B*48:01 was strongly associated with immediate reaction from BL hypersensitivity (OR = 37.4, 95%CI: 1.69-824.59; p = 0.016) while HLA-C*04:06, HLA-C*08:01 and HLA-DRB1*04:06 were associated with delayed reaction (p < 0.05). Among 71 cases who were newly evaluated for BL hypersensitivity, only 7 cases (9.8%) had true BL hypersensitivity.
Conclusions: Less than 10% of children with suspected of BL hypersensitivity have true hypersensitivity. There might be a role of HLA-B, HLA-C and HLA-DRB1 genotype in predicting BL hypersensitivity in Thai children.
Link to Academic article: DOI: 10.12932/AP-271118-0449
Journal : Asian Pacific journal of allergy and immunology
Bibliography : Singvijarn, P., Manuyakorn, W., Mahasirimongkol, S., Wattanapokayakit, S., Inunchot, W., Wichukchinda, N., Suvichapanich, S., Kamchaisatian, W., & Benjaponpitak, S. (2019, Apr 23). Association of HLA genotypes with Beta-lactam antibiotic hypersensitivity in children. Asian Pac J Allergy Immunol. doi: 10.12932/AP-271118-0449. Epub ahead of print. PMID: 31012593.
Cerebrovascular disease, risk faction, and quality of life: A systematic review (2019)
ชื่อบทความ : โรคหลอดเลือดสมอง ปัจจัยเสี่ยงและผลกกระทบต่อคุณภาพชีวิต
Title : Cerebrovascular disease, risk faction, and quality of life: A systematic review
ผู้เขียน/Author : วราภรณ์ คำรศ และ ทศพร เอกปรีชากุล
Researcher : Waraporn Khumros* and Thosporn Ekpreechakul
Department : * Faculty of Nursing, Siam University, Bangkok, Thailand
E-mail : wara_aui@hotmail.com
Abstract : Background: Cerebrovascular disease is a common neurological disease. However, these results remain unclear and can be awared of the factors that cause disease from everyday life. Researchers are interested in the subject. Objectives: This study aimed to assess the association between cerebrovascular disease and quality of life. Methods: A systematic review search of databases that met the inclusion and exclusion criteria. Relevant data were obtained from PubMed in 2009 to 2014. A systematic search of databases resulted in PICOS (Participants: stroke patients; Intervention: stroke; Comparison: control group, compare group; Outcome: association between cerebrovascular disease and quality of life and Study design: systematic review, meta-analysis, cohort study, literature review). Results: A systematic search of databases revealed that 85 articles met the inclusion and exclusion criteria from 92,790 articles. The result showed that cerebrovascular disease was associated with quality of life. Conclusion: This study suggested that cerebrovascular disease was associated with factor preventive disease. The knowledge can be applied in everyday life to reduce the number of cerebrovascular disease patients in the future
Keywords : Cerebrovascular disease, quality of life, risk factor of cerebrovascular disease
Donwload PDF : Cerebrovascular disease, risk faction, and quality of life: A systematic review
Link to Published: Chulalongkorn Medical Bulletin
Bibliography : วราภรณ์ คำรศ และ ทศพร เอกปรีชากุล. (2562). โรคหลอดเลือดสมอง ปัจจัยเสี่ยงและผลกกระทบต่อคุณภาพชีวิต Cerebrovascular disease, risk faction, and quality of life : A systematic review. Chula Med Bull, 1(5), 473-487.
Development of cycloguanil and pyrimethamine derivative as dihydrofolate reductase inhibitors (2019)
ผู้เขียนบทความ: ดร. วรท โชติปฏิเวชกุล และ พ.ญ อัญญา โชติปฏิเวชกุล
บทคัดย่อ:
มาลาเรียเป็นปัญหาที่สำคัญปัญหาหนึ่งที่พบบนโลกเพราะมีประชากรที่ติดเชื้อและตายด้วยโรคนี้มากว่าล้านคนต่อปี ซึ่งสาเหตุการติดเชื้อนี้เกิดจากเชื้อโปรโตซัวสายพันธุ์ Plasmodium โดยเฉพาะเชื้อสายพันธุ์ Plasmodium falciparum โดย pyrimethamine และ cycloguanil ถูกนำมาใช้ในการรักษาโรคไข้มาลาเรีย แต่กลับพบว่าค่าการออกฤทธิ์ยับยั้ง PfDHFR ของยานั้นมีค่าลดลงเนื่องจากการกลายพันธุ์ของ PfDHFR ดังนั้นจึงเป็นที่มาของการศึกษาอนุพันธ์ของ pyrimethamine และ cycloguanil เพื่อเป็นแนวหาตัวยับยั้งใหม่ที่สามารถออกฤทธิ์ยับยัง PfDHFR
คำสำคัญ: มาลาเรีย, dihydrofolate reductase, pyrimethamine, cycloguanil
Link to Academic article: Development of cycloguanil and pyrimethamine derivative as dihydrofolate reductase inhibitors
Doripenem dosing regimens in Asian critically ill patients with continuous renal replacement therapy (2019)
Title : Doripenem dosing regimens in Asian critically ill patients with continuous renal replacement therapy (2019)
Researcher : Chaijamorn, W., Puchsaka, P.W., Pattharachayakul, S., …Boonpeng, A., Pummangura, C.
Abstract : Antibiotic dosing in critically ill patients with continuous renal replacement therapy (CRRT) is still challenging. Pharmacokinetic changes in critically ill patients such as increased volume of distribution and decreased protein binding affinity affect hydrophilic drug dosing regimens [1]. Consequently, we might prescribe inadequate doses of antimicrobial agents in patients with CRRT [1] which can affect the morbidity and mortality associated with sepsis [2]. Requirement of loading dose and higher maintenance doses for this group of patients has been suggested to achieve pharmacokinetic and pharmacodynamic targets [3].
Continuous renal replacement therapy has been utilizing to effectively remove fluid and waste products in this group of patients due to hemodynamic instability [4]. Doripenem is a water-soluble carbapenem antibiotic and commonly used for Gram negative infection in intensive care unit (ICU) [5]. It can be removed by CRRT due to small molecular weight (438.52 Da) and low volume of distribution (16.8 L) [6].
The recommended dosing regimens from clinical resources are mostly from the pharmacokinetic studies in Western patients and there were a few studies conducted in Asian population [[7], [8], [9], [10], [11], [12]]. No suggested doripenem dosing regimens for CRRT patients based on Asian pharmacokinetic parameters exists. This study aimed to define the optimal doripenem dosing regimens using pharmacokinetic parameters from Asian population and body weights of Asian critically ill patients and to evaluate the probability of target attainment (PTA) of recommended dosing regimens from available clinical resources.
Link to article: https://doi.org/10.1016/j.jcrc.2019.04.030
Journal : ,
Bibliography : Chaijamorn, W., Puchsaka, P., Pattharachayakul, S., Charoensareerat, T., Srisawat, N., Boonpeng, A., & Pummangura, C. (Doripenem dosing regimens in Asian critically ill patients with continuous renal replacement therapy.
Effect of vitamin D on lung function assessed by forced oscillation technique in asthmatic children with vitamin D deficiency: A randomized double-blind placebo-controlled trial (2019)
Title : Effect of vitamin D on lung function assessed by forced oscillation technique in asthmatic children with vitamin D deficiency: A randomized double-blind placebo-controlled trial
Researcher : Clin.Prof.Suwat Benjaponpitak
Department : Faculty of Medicine, Siam University, Bangkok, Thailand
E-mail : med@siam.edu
Abstract : Background: The role of vitamin D and asthma in pulmonary function changes showed conflicting result.
Objective: To evaluate if vitamin D treatment would improve lung function assessed by forced oscillation technique (FOT) in vitamin D deficient asthmatic children.
Methods: A randomized double-blind placebo-controlled trial was performed in children, aged 3-18 years with well controlled asthma. Serum total 25(OH)D and FOT parameters including respiratory resistance at 5 Hz (R5), at 20 Hz (R20), respiratory reactance at 5 Hz (X5) and area of reactance (ALX), resonance frequency (Fres) were evaluated at baseline, 1 month and 3 months. Vitamin D deficient patients (serum total 25(OH)D < 20 ng/ml) were randomized to receive treatment with vitamin D2 (tVDD) or placebo (pVDD). Non-vitamin D deficient patients (nVDD) received placebo as a control group.
Results: A total of 84 children were recruited, 43 patients in nVDD group, 20 in tVDD group and 21 in pVDD group. There were no significant differences in age, sex, height and weight among groups. There were no significant differences of FOT parameters among groups at all visits. There was a trend toward decrease in R5/R20 from baseline to 1 month and 3 months visit in all groups, but the statistically significant improvement was observed only in nVDD group. Serum 25(OH)D showed no correlation with % predicted of FOT measures.
Conclusion: Vitamin D treatment in asthmatic children who had vitamin D deficiency may have no short term beneficial effect on pulmonary function assessed by FOT. Vitamin D supplementation in all asthmatic patient needs further study.
Link to Academic article: DOI: 10.12932/AP-010519-0553
Journal : Asian Pacific journal of allergy and immunology
Bibliography : Swangtrakul, N., Manuyakorn, W., Mahachoklertwattana, P., Kiewngam, P., Sasisakulporn, C., Jotikasthirapa, W., Kamchaisatian, W., & Benjaponpitak, S. (2019, December 14). Effect of vitamin D on lung function assessed by forced oscillation technique in asthmatic children with vitamin D deficiency: A randomized double-blind placebo-controlled trial. Asian Pac J Allergy Immunol, doi: 10.12932/AP-010519-0553. Epub ahead of print. PMID: 31837209.
Effectiveness of modified health belief model-based intervention to reduce body mass index for age in overweight junior high school students in Thailand (2019)
Title : Effectiveness of modified health belief model-based intervention to reduce body mass index for age in overweight junior high school students in Thailand
Researcher : Waraporn Kamrot*, Arnond Vorayingyong, Siriluck Suppapitiporn, Thanapoom Rattananupong, Vitool Lohsoonthorn
Department : * Faculty of Nursing, Siam University, Bangkok, Thailand
E-mail : wara_aui@hotmail.com
Link to Published: Journal of Health Research
Bibliography : Khumros, W., Vorayingyong, A., Rattananupong, T., Suppapitipornand, S. & Lohsoonthorn, V. (2019). Effectiveness of Modified Health Belief Model-Based Intervention to Reduce Body Mass Index for Age in Overweight Junior High School Students in Thailand. Journal of Health Research, 33(2), 162-172.
Effects of astaxanthin from shrimp shell on oxidative stress and behavior in animal model of Alzheimer’s disease (2019)
Title : Effects of astaxanthin from shrimp shell on oxidative stress and behavior in animal model of Alzheimer’s disease
Researcher : Taksima, T., Chonpathompikunlert, P., Sroyraya, M., Hutamekalin, P., Limpawattana, M., & Klaypradit, W.
Department : สำนักอธิการบดี มหาวิทยาลัยสยาม
E-mail : maruj.lim@siam.edu
ฐานข้อมูลงานวิจัย มหาวิทยาลัยสยาม : –
Link to article: Marine Drugs, 2019, 17(11), 628. https://doi.org/10.3390/md17110628
Journal : Marine Drugs / in Scopus
Bibliography : Taksima, T., Chonpathompikunlert, P., Sroyraya, M., Hutamekalin, P., Limpawattana, M., & Klaypradit, W. (2019). Effects of Astaxanthin from Shrimp Shell on Oxidative Stress and Behavior in Animal Model of Alzheimer’s Disease. Marine Drugs, 17(11), 628. https://doi.org/10.3390/md17110628
Factors affecting exercise behavior of subjects in Phasi Charoen District, Bangkok (2019)
ชื่อบทความ : ปัจจัยที่มีผลต่อพฤติกรรมการออกกำลังกายของกลุ่มตัวอย่างในเขตภาษีเจริญ กรุงเทพมหานคร
Title : Factors affecting exercise behavior of subjects in Phasi Charoen District, Bangkok
ผู้เขียน/Author : วราภรณ์ คำรศ, ชนิดา มัททวางกูร, ชัยสิทธิ์ ทันศึก
Researcher : Waraporn Khumros* Chanida Mattavangkul and Chaisit Thansuk
Department : * Faculty of Nursing, Siam University, Bangkok, Thailand
E-mail : wara_aui@hotmail.com
Abstract : Background: Daily activities affect health behavior which are importantly related to health problems in the society. People have less exercise and the application of local wisdom to promote exercise are limited in Bangkok, Thailand. Objectives: To study factors that affect exercise behavior of subjects in Phasi Charoen District. Methods: This cross-sectional descriptive study was designed. Data were collected using questionnaires to assess factors that affect exercise behavior, Cronbach’s alpha coefficient was 0.8988. The population sample were used in Phasi Charoen subjects. The accidential sampling data were collected from 404 subjects by comparing the rule of three in arithmetic among seven sub-districts. The data were analyzed with descriptive statistics such as percentage, mean, standard deviation, correlation analysis, t – test and F – test. Results: The results showed that attitude towards exercise, exercise awareness and supporting factors were statistically correlated with the behavior and exercise audibility directs (P < 0.01). Conclusion: This study access to the exercise of the Phasi Charoen District using qualitative research process. The data were collected by interviews and exercise behaviors and access should be facilitated in the other districts. Further studies could find ways to promote the fitness of the population in Thailand.
Keywords : Behavior exercise, exercise, factor, people in Phasi Charoen District.
Donwload PDF : Factors affecting exercise behavior of subjects in Phasi Charoen District, Bangkok
Link to Published: Chulalongkorn Medical Bulletin
Bibliography : วราภรณ์ คำรศ, ชนิดา มัททวางกูร และ ชัยสิทธิ์ ทันศึก. (2562). ปัจจัยที่มีผลต่อพฤติกรรมการออกกำลังกายของกลุ่มตัวอย่างในเขตภาษีเจริญ กรุงเทพมหานคร Factors affecting exercise behavior of subjects in Phasi Charoen District, Bangkok. Chula Med Bull, 1(4), 359-368.
Giant cutaneous turbid bullae in a 6-year-old boy with acute fever (2019)
Title : Giant cutaneous turbid bullae in a 6-year-old boy with acute fever
Researcher : Chiawsirikajorn, Y., Leelarasamee, A.
Department :
Abstract : A 6-year-old healthy Thai boy presented with multiple vesicles and bullae varying in size on the whole body, mostly on face and trunk for five days. The first vesicle arose on scalp while he had low-grade fever and felt headache on that day. A few days later, some vesicles progressed to large bullae containing turbid fluid or yellowish pus. Oral and genital mucosa were not involved.
Keywords: Varicella Bullosa, Bullous Chickenpox
Link to Academic article: http://www.jmatonline.com/index.php/jmat/article/view/9880
Journal : ,
Bibliography : Chiawsirikajorn, Y., & Leelarasamee, A. (2019). Giant cutaneous turbid bullae in a 6-year-old boy with acute fever.