Title           : Improvement of organic red pigment prodcution by Monascus Purpureus TISTR3651 using pathumthani-1 rice-based medium in submerged and solid-state fermentation

Researcher       : Techaoei, S., Jarmkom, K., Dumrongphuttidacha, T., Khobjai, W.

Department     :

Abstract            :  Objective: This research is to study the production of natural red pigment by Monascus purpureus TISTR3615 in the submerged and solid-state
fermentation system using Pathumthani-1 rice as a carbon source.
Methods: The antioxidant activity of the red pigment was evaluated in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS radical scavenging assay,
and ferric-reducing antioxidant power (FRAP) assay, including total phenolic compound.
Results and Discussion: The maximum of red pigment production was 0.55±0.02/ml (OD 680 nm) after incubation at 30°C for 24 days. The
antioxidant activity based on inhibition DPPH (%), ABTS radical scavenging activity (%), and FRAP activity (mM Fe2+/g) was 97.80±1.51,
68.64±0.46, and 0.32±0.021, respectively. The total phenolic content was 164.78±2.82 μg GAE/mg.
Conclusion: It was estimated that Monascus pigments, leading to nutraceutical and pharmaceutical applications, cosmetic industry, and food
industry.

Keywords: Natural pigment, Monascus purpureus, Submerged fermentation, Solid-state fermentation, Agricultural product

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0103

Journal : International Journal of Applied Pharmaceutics, 2021, 13(Special issue 1)

Bibliography     :  Techaoei, S., Jarmkom, K., Dumrongphuttidacha, T., & Khobjai, W. (2021). Improvement of organic red pigment prodcution by Monascus Purpureus TISTR3651 using pathumthani-1 rice-based medium in submerged and solid-state fermentation. International Journal of Applied Pharmaceutics, 13(Special issue 1), 47–50. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0103

Title           : In vitro antiplatelet and anticoagulant activity of indigenous vegetables from Southern Thailand

Researcher       : Sukati, S., Khobjai, W.

Department     :

Abstract            :  Objective: Epidemiological studies have indicated that diets rich in fruits and vegetables help reduce the risk of cardiovascular diseases (CVDs). However, data about the antithrombotic activity of local vegetables is rare. The objective of this study was to evaluate antiplatelet and anticoagulant activity in indigenous vegetables with high phenolic compounds collected from Southern Thailand.
Methods: Five selected indigenous vegetables were crudely extracted by distilled water and 80% methanol. The extracts were screened for in vitro antiplatelet and anticoagulant activity at a concentration of 10 μg/μl. The antiplatelet activity was measured by inhibition of platelet adhesion to collagen and thrombin-induced platelet aggregation, while the anticoagulant activity was assessed by the prothrombin time (PT) and activated partial thromboplastin time (APTT) tests.
Results: Among the selected vegetables, the extracts of mon-pu (Glochidion perakense Hook.f.) and young cashew leaves (Anacardium occidentale L.) showed high antithrombotic properties. The highest antithrombotic activity was observed in the methanolic extract of mon-pu, which showed
92.79±0.78% of platelet adhesion inhibition, 102.9±1.53% of platelet aggregation inhibition, and a prolonged APTT assay (48.92±0.94 s). The prolonged APTT but normal PT results suggested that the extract could affect factors VIII, IX, XI, and XII of the intrinsic coagulation pathway.
Conclusion: Our findings demonstrated antiplatelet and anticoagulation properties of indigenous vegetables from Southern Thailand. The multipotential effects of mon-pu extracts on antithrombosis evidently suggest that mon-pu can be considered as an excellent nutraceutical option in the prevention of thrombosis-related CVDs caused by different mechanisms.

Keywords: Indigenous vegetables, Antiplatelet activity, Anticoagulant activity, Southern Thailand

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0100

Journal : International Journal of Applied Pharmaceutics, 2021, 13(Special issue 1)

Bibliography     : Sukati, S., & Khobjai, W. (2021). In vitro antiplatelet and anticoagulant activity of indigenous vegetables from Southern Thailand. International Journal of Applied Pharmaceutics, 13(Special Issue 1), 38–42. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0100

Title           : Modulation of platelet functions by Careya sphaerica Roxb. leave extracts

Researcher       : Warachate Khobjai¹, Wanvisa Ninlaor², Worawan Watcharasamphankul³, Thaksaorn Thongom², Suriyan Sukati⁴

Department     : ¹ Department of Preclinic, Faculty of Medicine, Siam University, Phasi Charoen, Bangkok, Thailand
² Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
³ Department of Clinical Microbiology, Faculty of Medical Technology, Nation University, Lampang, Thailand
⁴ Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand

Abstract            :  Platelets form a plug to prevent blood loss and contribute to wound healing. Kradonbok, Careya sphaerica Roxb., is a Thai plant with medicinal properties. Conventionally, leaves of C. sphaerica are being used to help wound healing in Thailand. The present study was aimed to investigate the effect of C. sphaerica on the function of platelet. Four different extracts of leaves of C. sphaerica (distilled water, methanol, ethanol, and chloroform extracts) were prepared. The extracts at 5.0 mg/ml per dose were tested for the effect of C. sphaerica on platelet adhesion and aggregation properties, by employing a microtiter plate approach. The phytochemical identification was done by using gas chromatography–mass spectrometry (GC-MS). Our data revealed that chloroform extract significantly activated thrombin-induced platelet adhesion (105.27 ± 0.11%, P < 0.05). None of the extracts exhibited an improvement in platelet aggregation. Further GC-MS analysis of the chloroform extract revealed five key phytochemical constituents with potential platelet activation properties. In conclusion, our study evaluated platelet activation and potentially wound healing property of C. sphaerica. GC-MS analysis identified potential bioactive phytochemical compounds in C. sphaerica which warrant further investigation to characterize these compounds.

Keywords: Careya sphaerica roxb, platelet adhesion, platelet aggregation, primary hemostasis, wound healing

Link to Academic article:  DOI:10.4103/japtr.japtr_95_21  

Journal : Journal of Advanced Pharmaceutical Technology and Research, 2021, 12(4).

Bibliography     :  Khobjai, W., Ninlaor, W., Watcharasamphankul, W., Thongom, T., & Sukati, S. (2021). Modulation of platelet functions by Careya sphaerica Roxb. leave extracts. J Adv Pharm Technol Res, 12(4), 420-424. Retrieved from: https://www.japtr.org/text.asp?2021/12/4/420/328638

Title           : Phytonutrient screening by gas chromatography–mass spectrometry and anti-aging properties of glycosmis pentaphylla (Retz.) dc. (som-chuen) extracts

Researcher       : Dumrongphuttidecha, T., Khobjai, W., Techaeoi, S., Jarmkom, K., Thungmungmee, S.

Department     :

Abstract            :  Objective: The aims of this study were to screen the phytonutrient constituents and investigate their anti-aging property of leaves and bark of
Glycosmis pentaphylla (Retz.) DC. (GP).
Methods: GP is the medicinal plant which used as traditional medicine. Glycosmis pentaphylla leaves (GPL) and Glycosmis pentaphylla bark (GPB) of
GP were extracted with ethanol. The gas chromatography–mass spectrometry was used for phytonutrients analysis. The anti-aging properties were
performed using ABTS assay, deoxyribose degradation assay, bovine serum albumin (BSA)-fructose model, and matrix metalloproteinase 1 (MMP-1)
inhibitory activity.
Results: The important phytonutrients of GPL were α-tocopherol, linolenic acid, squalene, stigmasterol, and β-amyrin and for GPB were α-tocopherol,
phytol, campesterol, stigmasterol, dictamine, and γ-sitosterol. The percentage inhibition of GPL and GPB extracts at various concentrations was
between 16.57–76.05 and 20.66-78.81 by ABTS assay, 68.24–90.06 and 73.83–96.64% by deoxyribose degradation assay, 4.24–99.98 and 54.81–
99.94 by BSA-fructose model, and 6.31–81.55 and 1.06–74.45 by MMP-1 inhibitory activity, respectively.
Conclusion: Leaves and bark extracts of GP (Retz.) DC. are good sources of important phytonutrients and have the potential in anti-aging property.

Keywords: Glycosmis pentaphylla (Retz) DC, Anti-aging, Phytonutrients, Anti-Glycation, Matrix Mettalloproteinase-1

Link to Academic article: DOI: https://doi.org/10.22159/ijap.2021.v13s1.Y0105

Journal : International Journal of Applied Pharmaceuticsthis, 2021, 13(Special issue 1)

Bibliography     :  Dumrongphuttidecha, T., Khobjai, W., Techaeoi, S., Jarmkom, K., & Thungmungmee, S. (2021). Phytonutrient screening by gas chromatography–mass spectrometry and anti-aging properties of glycosmis pentaphylla (Retz.) dc. (som-chuen) extracts. International Journal of Applied Pharmaceuticsthis, 13(Special Issue 1), 55–58. Retrieved from https://doi.org/10.22159/ijap.2021.v13s1.Y0105