Nongenomic action of aldosterone on colocalization of angiotensin II type 1 and type 2 receptors in rat kidney (2018)

Title           : Nongenomic action of aldosterone on colocalization of angiotensin II type 1 and type 2 receptors in rat kidney

Researcher       : Sinphitukkul, K., Manotham, K., Eiam-Ong, S., Eiam-Ong, S.

Abstract            :  Previous in vitro studies have demonstrated that angiotensin II type 1 and type 2 receptors (AT₁R and AT₂R) are co-localized and can form AT₁R/AT₂R dimerization in rat proximal tubular cells. Aldosterone non-genomically enhances angiotensin II receptor dimerization. We found no other in vivo studies in the literature regarding the effect of aldosterone on colocalization of AT₁R and AT₂R in whole kidney. Male Wistar rats were intraperitoneally injected with either normal saline solution (sham group) or aldosterone (experimental group). Colocalization of renal AT₁R and AT₂R proteins was examined by double immunohistochemical staining. The colocalization of AT₁R and AT₂R proteins was more prominent in the glomerulus, distal convoluted tubules, and cortical collecting ducts while colocalization was weak and diffused in the proximal convoluted tubules and peritubular capillaries in both groups. Our in vivo study showed aldosterone did not alter a constitutive colocalization of AT₁R and AT₂R proteins in the renal cortex and medulla. However, these proteins were colocalized more prominently in the renal cortex.

Keywords: Aldosterone, angiotensin II receptors, nongenomic action, protein colocalization, rat kidney

Link to Academic article: https://doi.org/10.1080/01478885.2018.1438756

Journal : Journal of Histotechnology, 2018, 41(2).

Bibliography     : Sinphitukkul, K., Manotham, K., Eiam-Ong, S., & Eiam-Ong, S. (2018). Nongenomic action of aldosterone on colocalization of angiotensin II type 1 and type 2 receptors in rat kidney. Journal of Histotechnology, 41(2), 58–65.